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Strep-Spin Protein Miniprep Kit, 10 preps

Price$ 82.00
  • SKU: ZP2004
  • Pack Size: 10 preps
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Strep-Spin Protein Miniprep Kit, 10 preps

2-8°C


The Strep-Spin Protein Miniprep Kit provides a fast spin-column based purification technology for Strep-tagged proteins. Up to 450 µg of Strep-tagged protein can be purified in 7 minutes and eluted in Strep-Elution Buffer. The purified protein is ultra-pure and can be used directly for enzymatic assays, protein biochemical analyses, SDS-PAGE and other sensitive applications. The straightforward spin, wash, and elute protocol dramatically simplifies protein purification and allows the end user to get results in minutes, not hours.

Fast: Purify Strep-tagged proteins from cell lysates in less than 7 minutes.
Simple: Prepare pure protein for small-scale studies using a spin-column.
Convenient: No special instrumentation needed other than a benchtop microcentrifuge.

1 lbs

4.25 x 3.00 x 3.00



Research or further manufacturing use only, not for food or drug use.
Affinity Matrix: Strep-Tactin XT Superflow 50%
Binding Capacity: 100 µl of Strep-Tactin XT Superflow 50% suspension can bind 15 nmol biotin (approx. 450 µg of a 30 kDa Twin-Strep-tag protein).
Elution Method: Biotin excess
Elution Volume: Recommended 3 x 200 µl
Principle of Technology: The Strep-Spin Protein Miniprep Kit is based on a novel StrepTactin XT Superflow resin which binds to Twin Strep-tag with very high affinity. This tag contains two Strep-tag II peptides connected via a Glycine/Serine linker sequence. Hence this kit is optimized for the purification of Twin-Step-tagged proteins; however it will also work efficiently for proteins with single Strep-tags.
Processing Time: 7 minutes
Product Storage: Please store the Strep-Tactin XT Superflow 50% suspension, Strep-Wash Buffer, and Strep-Elution Bufer at 4°C. The other components can be stored at room temperature.
Protein Purity: Electrophoretically pure. Purified high-quality protein is suitable for enzyme kinetics, protein biochemical analyses, SDS-PAGE, and other applications.
Required Equipment: Microcentrifuge
Sample Type: Cell lysates or other complex protein mixtures containing Strep-tagged proteins.

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