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MBP-Spin Protein Miniprep Kit, 10 Preps

Price$ 82.00
  • SKU: ZP2006
  • Pack Size: 10 preps
MBP-Spin Protein Miniprep Kit, 10 Preps

Room Temperature

The MBP-Spin Protein Miniprep Kit provides a fast spin-column based purification technology for MBP-tagged proteins. Up to 1000 µg of MBP-tagged protein can be purified in 6 minutes and eluted in MBP-Elution Buffer. The purified protein is ultra-pure and can be used directly for enzymatic assays, biochemical analyses, SDS-PAGE and other sensitive applications. The straightforward spin, wash, and elute protocol dramatically simplifies protein purification and allows the end user to get results in minutes, not hours.

Fast: Purify MBP-tagged proteins from cell lysates in less than 6 minutes.
Simple: Prepare pure protein for small-scale studies using a spin-column.
Convenient: No special instrumentation needed other than a benchtop microcentrifuge.

1 lbs

4.00 x 4.00 x 6.00

Research or further manufacturing use only, not for food or drug use.
Affinity Matrix: Amylose Resin
Binding Capacity: 100 µl of Amylose Resin typically binds ≥ 400 µg of fusion protein.
Elution Method: Excess Maltose
Elution Volume: ≥ 200 µl
Principle of Technology: The MBP-Spin Protein Miniprep Kit uses an affinity matrix composed of amylose resin to specifically bind proteins fused to maltose-binding protein (MBP). MBP is known to significantly enhance the solubility of many proteins when fused to them.
Processing Time: 6 minutes
Product Storage: Please store the Amylose Resin at 4 °C. The other components can be stored at room temperature.
Purity: Electrophoretically pure and suitable for enzyme kinetics, biochemical analyses, SDS-PAGE, and other applications.
Required Equipment: Microcentrifuge
Sample Type: Cell lysates or other complex protein mixtures containing MBP-tagged proteins.

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